OriDB Curated Paper

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Genome-wide localization of pre-RC sites and identification of replication origins in fission yeast.

Makoto Hayashi, Yuki Katou, Takehiko Itoh, Atsutoshi Tazumi, Mitsutoshi Tazumi, Yoshiki Yamada, Tatsuro Takahashi, Takuro Nakagawa, Katsuhiko Shirahige, Hisao Masukata

EMBO J. (2007), 26(5):1327-39Data to DownloadPubMed | PubMed Central | EMBO J.

DNA replication of eukaryotic chromosomes initiates at a number of discrete loci, called replication origins. Distribution and regulation of origins are important for complete duplication of the genome. Here, we determined locations of Orc1 and Mcm6, components of pre-replicative complex (pre-RC), on the whole genome of Schizosaccharomyces pombe using a high-resolution tiling array. Pre-RC sites were identified in 460 intergenic regions, where Orc1 and Mcm6 colocalized. By mapping of 5-bromo-2'-deoxyuridine (BrdU)-incorporated DNA in the presence of hydroxyurea (HU), 307 pre-RC sites were identified as early-firing origins. In contrast, 153 pre-RC sites without BrdU incorporation were considered to be late and/or inefficient origins. Inactivation of replication checkpoint by Cds1 deletion resulted in BrdU incorporation with HU specifically at the late origins. Early and late origins tend to distribute separately in large chromosome regions. Interestingly, pericentromeric heterochromatin and the silent mating-type locus replicated in the presence of HU, whereas the inner centromere or subtelomeric heterochromatin did not. Notably, MCM did not bind to inner centromeres where origin recognition complex was located. Thus, replication is differentially regulated in chromosome domains.

OriDB annotation of this paper:

ARS assay

None curated.

2D gel

None curated.

ChIP of replication origin proteins

ARS at I-15, ARS at I-51, ARS at I-54, ARS at I-62, ARS at I-70, ARS at I-74, ARS at I-79, ARS at I-85, ARS at I-88, ARS at I-95, I-113, ARS at I-150, ARS at I-166, ARS at I-190, ARS at I-199, ARS at I-239, ARS at I-244, ARS at I-294, ARS at I-302, ARS at I-365, ARS at I-410, ARS at I-440, ARS at I-457, ARS at I-464, ARS at I-474, ARS at I-490, ARS at I-563, ARS at I-592, ARS at I-608, ARS at I-650, I-712, ARS at I-740, ARS at I-750, ARS at I-761, ARS at I-765, ARS at I-784, ARS at I-794, ARS at I-844, ARS at I-893, ARS at I-902, ARS at I-938, ARS at I-965, I-1028, ARS at I-1080, ARS at I-1083, ARS at I-1090, I-1095, ARS at I-1148, ARS at I-1157, ARS at I-1162, ARS at I-1191, ARS at I-1196, ARS at I-1225, I-1252, ARS at I-1275, ARS at I-1360, ARS at I-1444, ARS at I-1476, ARS at I-1499, ARS at I-1509, ARS at I-1574, ARS at I-1599, ARS at I-1667, ARS at I-1708, ARS at I-1719, ARS at I-1766, ARS at I-1823, ARS at I-1834, ARS at I-1892, ARS at I-1895, ARS at I-1946, ARS at I-1954, ARS at I-1958, ARS at I-1975, ARS at I-2025, ARS at I-2042, ARS at I-2096, ARS at I-2140, ARS at I-2194, I-2226, ARS at I-2232, ARS at I-2250, ARS at I-2263, ARS at I-2274, ARS at I-2280, ARS at I-2304, ARS at I-2339, ARS at I-2363, ARS at I-2406, ARS at I-2416, I-2439, ARS at I-2442, ARS at I-2452, ARS at I-2457, ARS at I-2510, ARS at I-2525, ARS at I-2528, ARS at I-2578, ARS at I-2600, ARS at I-2615, ARS at I-2618, ARS at I-2643, ARS at I-2668, ARS at I-2719, ARS at I-2761, ARS at I-2803, ARS at I-2875, ARS at I-2883, ARS at I-2938, ARS at I-2960, ARS at I-2977, ARS at I-2980, ARS at I-2983, ARS at I-3010, ARS at I-3022, ARS at I-3032, ARS at I-3053, I-3060, ARS at I-3070, ARS at I-3127, ARS at I-3144, ARS at I-3158, ARS at I-3184, ARS at I-3301, ARS at I-3321, ARS at I-3405, ARS at I-3410, I-3501, ARS at I-3541, ARS at I-3549, ARS at I-3570, ARS at I-3577, ARS at I-3579, ARS at I-3592, ARS at I-3611, ARS at I-3635, ARS at I-3646, ARS at I-3700, ARS at I-3747, ARS at I-3753, ARS at I-3840, ARS at I-3852, ARS at I-3878, ARS at I-3882, ARS at I-3898, I-3954, ARS at I-3992, ARS at I-3995, ARS at I-4009, ARS at I-4019, I-4073, ARS at I-4088, ARS at I-4136, ARS at I-4140, ARS at I-4154, ARS at I-4177, ARS at I-4180, ARS at I-4185, ARS at I-4197, ARS at I-4232, ARS at I-4239, I-4255, ARS at I-4275, ARS at I-4326, ARS at I-4365, ARS at I-4386, ARS at I-4415, ARS at I-4439, ARS at I-4450, ARS at I-4458, ARS at I-4468, ARS at I-4484, ARS at I-4533, ARS at I-4605, ARS at I-4653, ARS at I-4674, ARS at I-4739, ARS at I-4779, ARS at I-4803, ARS at I-4844, ARS at I-4853, ARS at I-4882, ARS at I-4912, ARS at I-4917, ARS at I-4963, ARS at I-4984, I-5025, ARS at I-5097, ARS at I-5156, ARS at I-5178, ARS at I-5189, ARS at I-5234, ARS at I-5271, ARS at I-5322, ARS at I-5393, ARS at I-5449, ARS at I-5469, ARS at I-5476, ARS at I-5482, ARS at I-5487, ARS at I-5501, ARS at I-5506, ARS at I-5510, ARS at I-5516, ARS at I-5521, ARS at I-5529, II-32, ARS at II-38, ARS at II-53, ARS at II-63, ARS at II-66, ARS at II-70, ARS at II-75, ARS at II-78, ARS at II-81, ARS at II-86, ARS at II-90, ARS at II-104, ARS at II-111, ARS at II-117, ARS at II-132, ARS at II-141, ARS at II-151, ARS at II-201, ARS at II-204, ARS at II-228, ARS at II-258, ARS at II-278, ARS at II-281, ARS at II-323, ARS at II-326, ARS at II-332, ARS at II-355, ARS at II-454, ARS at II-484, ARS at II-518, ARS at II-520, ARS at II-522, ARS at II-529, ARS at II-561, ARS at II-570, ARS at II-592, II-604, ARS at II-669, ARS at II-674, ARS at II-717, ARS at II-721, ARS at II-723, ARS at II-746, ARS at II-833, ARS at II-838, ARS at II-840, ARS at II-843, ARS at II-847, ARS at II-891, ARS at II-949, ARS at II-957, ARS at II-962, ARS at II-1055, ARS at II-1079, ARS at II-1105, ARS at II-1142, ARS at II-1148, ARS at II-1244, II-1254, ARS at II-1266, ARS at II-1269, II-1278, ARS at II-1313, ARS at II-1351, ARS at II-1366, ARS at II-1389, ARS at II-1444, ARS at II-1457, ARS at II-1461, ARS at II-1500, II-1547, ARS at II-1556, ARS at II-1589, ARS at II-1655, ARS at II-1666, ARS at II-1681, ARS at II-1706, ARS at II-1717, ARS at II-1750, ARS at II-1778, ARS at II-1805, ARS at II-1868, ARS at II-1893, ARS at II-1942, ARS at II-1953, ARS at II-1964, ARS at II-2023, ARS at II-2052, ARS at II-2070, II-2106, ARS at II-2111, II-2119, ARS at II-2129, ARS at II-2132, ARS at II-2137, ARS at II-2177, II-2187, ARS at II-2246, ARS at II-2266, ARS at II-2319, ARS at II-2339, ARS at II-2411, ARS at II-2443, ARS at II-2524, ARS at II-2531, ARS at II-2553, II-2582, ARS at II-2594, ARS at II-2613, ARS at II-2648, ARS at II-2702, ARS at II-2739, II-2791, II-2800, ARS at II-2829, ARS at II-2901, ARS at II-2910, ARS at II-2939, ARS at II-2987, ARS at II-3052, ARS at II-3098, ARS at II-3162, ARS at II-3176, ARS at II-3287, ARS at II-3338, II-3363, ARS at II-3379, ARS at II-3408, ARS at II-3422, ARS at II-3467, ARS at II-3488, ARS at II-3491, ARS at II-3502, ARS at II-3511, ARS at II-3513, ARS at II-3557, ARS at II-3597, ARS at II-3605, ARS at II-3635, ARS at II-3702, ARS at II-3720, ARS at II-3763, ARS at II-3778, ARS at II-3802, ARS at II-3815, ARS at II-3839, ARS at II-3916, ARS at II-3927, ARS at II-3938, ARS at II-3975, ARS at II-3985, ARS at II-4019, ARS at II-4031, ARS at II-4034, ARS at II-4107, ARS at II-4129, ARS at II-4203, ARS at II-4226, ARS at II-4237, ARS at II-4252, ARS at II-4292, ARS at II-4344, ARS at II-4360, ARS at II-4412, ARS at II-4420, ARS at II-4426, ARS at II-4437, ARS at II-4441, ARS at II-4452, ARS at II-4454, ARS at II-4457, ARS at III-35, ARS at III-77, III-123, ARS at III-189, ARS at III-207, ARS at III-232, ARS at III-254, ARS at III-279, ARS at III-287, ARS at III-306, ARS at III-348, ARS at III-370, ARS at III-398, ARS at III-505, ARS at III-519, ARS at III-565, ARS at III-575, ARS at III-606, ARS at III-643, ARS at III-666, ARS at III-720, ARS at III-764, ARS at III-827, ARS at III-848, ARS at III-854, ARS at III-937, ARS at III-967, ARS at III-972, ARS at III-986, ARS at III-1039, ARS at III-1066, ARS at III-1141, ARS at III-1253, ARS at III-1323, ARS at III-1342, ARS at III-1371, ARS at III-1373, ARS at III-1393, ARS at III-1396, III-1412, III-1417, ARS at III-1448, ARS at III-1511, ARS at III-1524, ARS at III-1531, ARS at III-1555, ARS at III-1576, ARS at III-1594, ARS at III-1609, ARS at III-1672, ARS at III-1675, ARS at III-1680, ARS at III-1685, ARS at III-1689, III-1719, ARS at III-1722, ARS at III-1727, ARS at III-1754, ARS at III-1814, III-1824, III-1839, III-1844, ARS at III-1860, ARS at III-1867, ARS at III-1878, ARS at III-1922, ARS at III-1929, ARS at III-1936, ARS at III-1969, ARS at III-2035, ARS at III-2037, ARS at III-2042, ARS at III-2046, ARS at III-2064, ARS at III-2068, ARS at III-2072, ARS at III-2076, ARS at III-2107, ARS at III-2201, ARS at III-2258, ARS at III-2276, ARS at III-2300, ARS at III-2309, ARS at III-2382

Replication timing

None curated.

Replication in hydroxyurea

None curated.

Predicted origins

None curated.

Confirmed sequence element

None curated.

Predicted sequence element

None curated.

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