OriDB Curated Paper

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Genome-wide characterization of fission yeast DNA replication origins.

Christian Heichinger, Christopher J Penkett, Jürg Bähler, Paul Nurse

EMBO J. (2006), 25(21):5171-9Data to DownloadPubMed | PubMed Central | EMBO J.

Eukaryotic DNA replication is initiated from multiple origins of replication, but little is known about the global regulation of origins throughout the genome or in different types of cell cycles. Here, we identify 401 strong origins and 503 putative weaker origins spaced in total every 14 kb throughout the genome of the fission yeast Schizosaccharomyces pombe. The same origins are used during premeiotic and mitotic S-phases. We found that few origins fire late in mitotic S-phase and that activating the Rad3 dependent S-phase checkpoint by inhibiting DNA replication had little effect on which origins were fired. A genome-wide analysis of eukaryotic origin efficiencies showed that efficiency was variable, with large chromosomal domains enriched for efficient or inefficient origins. Average efficiency is twice as high during mitosis compared with meiosis, which can account for their different S-phase lengths. We conclude that there is a continuum of origin efficiency and that there is differential origin activity in the mitotic and meiotic cell cycles.

OriDB annotation of this paper:

ARS assay

None curated.

2D gel

None curated.

ChIP of replication origin proteins

None curated.

Replication timing

None curated.

Replication in hydroxyurea

ARS at I-95, I-113, ARS at I-124, ARS at I-199, ARS at I-230, I-327, ARS at I-358, ARS at I-365, ARS at I-397, ARS at I-440, ARS at I-474, ARS at I-490, ARS at I-528, ARS at I-563, ARS at I-608, ARS at I-650, I-712, I-712, I-712, ARS at I-794, ARS at I-843, ARS at I-844, ARS at I-876, ARS at I-881, ARS at I-902, ARS at I-965, ARS at I-978, ARS at I-1021, ARS at I-1080, ARS at I-1083, ARS at I-1090, I-1095, ARS at I-1140, ARS at I-1157, ARS at I-1191, ARS at I-1196, I-1252, ARS at I-1275, ARS at I-1281, ARS at I-1374, ARS at I-1389, ARS at I-1421, ARS at I-1444, ARS at I-1444, ARS at I-1476, ARS at I-1488, ARS at I-1504, ARS at I-1504, ARS at I-1509, ARS at I-1574, ARS at I-1619, ARS at I-1665, ARS at I-1667, ARS at I-1708, ARS at I-1719, ARS at I-1734, ARS at I-1828, ARS at I-1834, ARS at I-1859, ARS at I-1975, ARS at I-2042, ARS at I-2044, ARS at I-2085, ARS at I-2096, ARS at I-2133, ARS at I-2133, ARS at I-2140, ARS at I-2207, ARS at I-2232, I-2226, ARS at I-2243, ARS at I-2245, ARS at I-2243, ARS at I-2245, ARS at I-2250, ARS at I-2263, ARS at I-2280, ARS at I-2310, ARS at I-2318, ARS at I-2336, ARS at I-2339, ARS at I-2406, ARS at I-2423, ARS at I-2442, I-2439, ARS at I-2452, ARS at I-2498, ARS at I-2578, ARS at I-2615, ARS at I-2618, ARS at I-2643, ARS at I-2672, ARS at I-2719, ARS at I-2746, ARS at I-2761, ARS at I-2785, ARS at I-2803, ARS at I-2815, ARS at I-2862, ARS at I-2883, ARS at I-2913, ARS at I-2960, ARS at I-2980, ARS at I-2983, ARS at I-3010, ARS at I-3022, I-3060, ARS at I-3127, ARS at I-3143, ARS at I-3144, ARS at I-3184, I-3247, ARS at I-3270, ARS at I-3301, ARS at I-3321, ARS at I-3405, ARS at I-3410, ARS at I-3443, ARS at I-3461, I-3501, ARS at I-3525, ARS at I-3549, ARS at I-3573, ARS at I-3577, ARS at I-3603, ARS at I-3635, ARS at I-3689, ARS at I-3700, ARS at I-3729, ARS at I-3736, ARS at I-3747, ARS at I-3753, ARS at I-3753, ARS at I-3756, ARS at I-3767, ARS at I-3772, ARS at I-3786, ARS at I-3812, ARS at I-3840, ARS at I-3852, ARS at I-3865, ARS at I-3874, ARS at I-3882, ARS at I-3898, I-3954, ARS at I-3992, ARS at I-3995, ARS at I-4019, I-4073, ARS at I-4088, ARS at I-4136, ARS at I-4140, ARS at I-4185, ARS at I-4197, I-4255, ARS at I-4275, ARS at I-4326, ARS at I-4415, ARS at I-4439, ARS at I-4484, ARS at I-4493, ARS at I-4517, ARS at I-4533, ARS at I-4575, ARS at I-4585, ARS at I-4605, ARS at I-4618, ARS at I-4658, ARS at I-4684, ARS at I-4722, ARS at I-4739, ARS at I-4779, ARS at I-4803, ARS at I-4844, ARS at I-4853, ARS at I-4874, ARS at I-4925, ARS at I-4934, ARS at I-4963, ARS at I-4978, I-5025, I-5046, ARS at I-5097, ARS at I-5140, ARS at I-5156, ARS at I-5165, ARS at I-5178, ARS at I-5234, ARS at I-5253, ARS at I-5322, ARS at I-5393, ARS at I-5436, ARS at I-5449, ARS at I-5476, ARS at I-5482, ARS at I-5487, ARS at I-5498, ARS at I-5501, ARS at I-5504, ARS at I-5555, ARS at I-5559, ARS at I-5569, ARS at I-5571, ARS at II-94, ARS at II-97, ARS at II-122, ARS at II-141, ARS at II-151, ARS at II-258, ARS at II-268, ARS at II-268, ARS at II-278, ARS at II-289, ARS at II-315, ARS at II-323, ARS at II-326, ARS at II-360, ARS at II-409, II-448, ARS at II-459, ARS at II-513, ARS at II-513, ARS at II-518, ARS at II-520, ARS at II-522, ARS at II-561, ARS at II-570, ARS at II-592, II-604, ARS at II-637, ARS at II-649, ARS at II-674, ARS at II-684, ARS at II-717, ARS at II-721, ARS at II-746, ARS at II-753, ARS at II-824, ARS at II-840, ARS at II-843, ARS at II-915, ARS at II-939, ARS at II-957, ARS at II-1020, ARS at II-1048, ARS at II-1059, ARS at II-1105, ARS at II-1142, ARS at II-1156, ARS at II-1170, ARS at II-1244, II-1254, ARS at II-1265, ARS at II-1266, ARS at II-1269, ARS at II-1269, II-1278, ARS at II-1307, ARS at II-1313, ARS at II-1389, ARS at II-1429, ARS at II-1444, ARS at II-1461, ARS at II-1531, II-1547, ARS at II-1589, ARS at II-1646, II-1625, ARS at II-1655, ARS at II-1666, ARS at II-1702, ARS at II-1717, ARS at II-1766, ARS at II-1778, ARS at II-1805, ARS at II-1868, ARS at II-1929, ARS at II-1942, ARS at II-1953, ARS at II-1964, ARS at II-2023, ARS at II-2036, ARS at II-2052, ARS at II-2070, II-2119, ARS at II-2129, ARS at II-2132, ARS at II-2137, ARS at II-2159, ARS at II-2177, II-2187, ARS at II-2206, ARS at II-2246, ARS at II-2319, ARS at II-2319, ARS at II-2374, ARS at II-2432, ARS at II-2443, ARS at II-2524, ARS at II-2553, ARS at II-2594, ARS at II-2601, ARS at II-2625, ARS at II-2673, ARS at II-2702, ARS at II-2739, ARS at II-2774, II-2791, II-2791, II-2800, ARS at II-2901, ARS at II-2939, ARS at II-2987, ARS at II-3052, ARS at II-3070, ARS at II-3098, ARS at II-3137, ARS at II-3176, ARS at II-3233, ARS at II-3287, ARS at II-3333, ARS at II-3338, II-3335, II-3358, II-3363, ARS at II-3418, ARS at II-3418, ARS at II-3422, ARS at II-3467, ARS at II-3488, ARS at II-3491, ARS at II-3511, ARS at II-3513, ARS at II-3557, ARS at II-3605, ARS at II-3635, ARS at II-3660, ARS at II-3681, ARS at II-3720, ARS at II-3754, ARS at II-3763, ARS at II-3802, ARS at II-3815, ARS at II-3826, ARS at II-3839, ARS at II-3898, ARS at II-3900, ARS at II-3916, ARS at II-3938, ARS at II-4019, ARS at II-4030, ARS at II-4031, ARS at II-4034, II-4141, ARS at II-4177, ARS at II-4203, ARS at II-4226, ARS at II-4237, ARS at II-4292, ARS at II-4360, ARS at II-4372, ARS at II-4412, ARS at II-4437, ARS at II-4452, ARS at II-4454, ARS at II-4469, ARS at III-35, ARS at III-54, ARS at III-69, ARS at III-69, ARS at III-77, ARS at III-109, III-120, III-123, III-120, III-123, ARS at III-189, ARS at III-207, ARS at III-232, ARS at III-248, ARS at III-254, ARS at III-279, ARS at III-287, ARS at III-315, ARS at III-332, ARS at III-370, ARS at III-398, ARS at III-473, ARS at III-505, ARS at III-519, ARS at III-528, ARS at III-558, ARS at III-565, ARS at III-575, ARS at III-606, ARS at III-616, ARS at III-643, ARS at III-666, ARS at III-720, ARS at III-764, ARS at III-766, ARS at III-821, ARS at III-854, ARS at III-906, ARS at III-937, ARS at III-967, ARS at III-972, ARS at III-986, ARS at III-1039, ARS at III-1059, ARS at III-1059, ARS at III-1066, ARS at III-1059, ARS at III-1066, ARS at III-1141, ARS at III-1194, ARS at III-1253, ARS at III-1323, ARS at III-1342, ARS at III-1371, ARS at III-1373, ARS at III-1396, III-1412, III-1417, ARS at III-1448, ARS at III-1511, ARS at III-1531, ARS at III-1555, ARS at III-1576, ARS at III-1594, ARS at III-1609, ARS at III-1657, ARS at III-1684, ARS at III-1685, ARS at III-1689, ARS at III-1722, III-1719, ARS at III-1722, ARS at III-1727, ARS at III-1754, ARS at III-1814, III-1824, III-1839, III-1844, ARS at III-1867, ARS at III-1884, ARS at III-1929, ARS at III-1969, ARS at III-1975, ARS at III-1975, ARS at III-2022, ARS at III-2035, ARS at III-2037, ARS at III-2068, ARS at III-2101, ARS at III-2107, ARS at III-2117, ARS at III-2140, ARS at III-2201, ARS at III-2219, ARS at III-2276, ARS at III-2300, ARS at III-2339, ARS at III-2364, ARS at III-2382, ARS at III-2415, ARS at III-2426, ARS at III-2429, ARS at III-2434

Predicted origins

None curated.

Confirmed sequence element

None curated.

Predicted sequence element

None curated.

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